Biomarquer analytical results from Cainozoic age organic rich lutites from Duero and Ebro basin drill holes are presented. The presence and abundance of n-alkane, n-metil ketones, n-etil-ketonés, n-butil ketones and elemental sulphur are compared. Samples from Ebro basin bore hole core (PE-1) reveal alternances of "terrestrial" and "acuatic" lacustrine environmental conditions (shallowing versus deepening conditions) while samples from Duero basin borehole core (VS-1) show a constant "terrestrial (shallow) character