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dc.contributor.authorGojkovic, Živan
dc.contributor.authorVílchez Lobato, Carlos 
dc.contributor.authorTorronteras Santiago, Rafael 
dc.contributor.authorVigara Fernández, Javier 
dc.contributor.authorGómez Jacinto, Verónica 
dc.contributor.authorJanzer, Nora
dc.contributor.authorGómez Ariza, José Luis 
dc.contributor.authorMárová, Ivana
dc.contributor.authorGarbayo Nores, Inés
dc.identifier.citationGojkovic, Ž., Vílchez Lobato, C., Torronteras Santiago, R., Vigara Fernández, J., Gómez Jacinto, V., Janzer, N., Gómez Ariza, J.L., Márová, I., Garbayo Nores, I.: "Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture". ScientificWorld Journal. Volume 2014 (2014), Article ID 401265. ISSN 1537-744X
dc.description.abstractThe aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg⋅L−1 (212 μ M) decreased growth rates for about 25% compared to control. A selenate EC50 value of 45mg⋅L−1 (238.2 μ M) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140mg⋅kg−1 of SeMet in 120h of cultivation.This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se.en_US
dc.publisherHindawi Publishing Corporationen_US
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.subject.otherChlorella sorokiniana
dc.titleEffect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Cultureen_US

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