A multi-tracer test was done in the Alta Cadena and Los Tajos mountains range (Malaga province, southern Spain) in order to delimitate the aquifer system drained by Villanueva del Rosario spring and to precise its hydrogeological behaviour. The results show a partitioning of the carbonate outcrops in different aquifer systems. The system drained by Villanueva del Rosario exhibits clear a karstic behaviour with flow velocities above 200 m/h. The karst conduits and the groundwater flow path in this system are determined by ENE-WSW folds and thrust, and transverse fractures