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dc.contributor.authorPérez, David
dc.contributor.authorBautista Pulido, María
dc.contributor.authorLeón Bañares, Rosa María 
dc.contributor.authorMolina Márquez, Ana
dc.contributor.authorVila Spinola, Marta 
dc.contributor.authorLópez López, Manuel 
dc.contributor.authorLópez Cornejo, Pilar
dc.identifier.citationPérez, D., Moyá, M. L., Bautista, M., León, R., Molina-Márquez, A., Vila, M., Romero-Azogil, L., Benito, E., de Gracia García-Martín, M., Moreno-Gordillo, P., Rosado, I. V., Balestra, F. R., Huertas, P., López-López, M., & López-Cornejo, P. (2023). A novel biocompatible polymer derived from D-mannitol used as a vector in the field of genetic engineering of eukaryotic cells. In Colloids and Surfaces B: Biointerfaces (Vol. 224, p. 113219). Elsevier BV.
dc.identifier.issn1873-4367 (electrónico)
dc.description.abstractThe design and preparation of new vectors to transport genetic material and increase the transfection efficiency continue being an important research line. Here, a novel biocompatible sugar-based polymer derived from Dmannitol has been synthesized to be used as a gene material nanocarrier in human (gene transfection) and microalga cells (transformation process). Its low toxicity allows its use in processes with both medical and industrial applications. A multidisciplinary study about the formation of polymer/p-DNA polyplexes has been carried out using techniques such as gel electrophoresis, zeta potential, dynamic light scattering, atomic force microscopy, and circular dichroism spectroscopy. The nucleic acids used were the eukaryotic expression plasmid pEGFP-C1 and the microalgal expression plasmid Phyco69, which showed different behaviors. The importance of DNA supercoiling in both transfection and transformation processes was demonstrated. Better results were obtained in microalga cells nuclear transformation than in human cells gene transfection. This was related to the plasmid’s conformational changes, in particular to their superhelical structure. It is noteworthy that the same nanocarrier has been used with eukaryotic cells from both human and microalga.es_ES
dc.description.sponsorshipThis work was supported by the Consejería de Conocimiento, Innovación y Universidades de la Junta de Andalucía (FQM-206, FQM-274, FQM-135 and P20–01234); VI Plan Propio Universidad de Sevilla (PP2019/00000748), and the European Union (Feder Funds).es_ES
dc.relation.isversionofPublisher’s version
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subject.otherGene therapyes_ES
dc.subject.otherTransformation process in algaees_ES
dc.subject.otherSupercoiling structurees_ES
dc.subject.otherBiocompatible polymeres_ES
dc.titleA novel biocompatible polymer derived from D-mannitol used as a vector in the field of genetic engineering of eukaryotic cellses_ES
dc.subject.unesco2302 Bioquímicaes_ES

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