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dc.contributor.authorCallejón Leblic, María Belén 
dc.contributor.authorRodríguez Moro, Gema 
dc.contributor.authorArias Borrego, Ana 
dc.contributor.authorPereira Vega, Antonio
dc.contributor.authorGómez Ariza, José Luis 
dc.contributor.authorGarcía Barrera, Tamara
dc.identifier.citationCallejón-Leblic, B., Rodríguez-Moro, G., Arias-Borrego, A., Pereira-Vega, A., Gómez-Ariza, J. L., & García-Barrera, T. (2020). Absolute quantification of selenoproteins and selenometabolites in lung cancer human serum by column switching coupled to triple quadrupole inductively coupled plasma mass spectrometry. Journal of Chromatography A, 1619, 460919.
dc.description.abstractOne of the most important causes of the high mortality rate and low life expectancy of lung cancer is the detection at advanced stages. Thus, there is an urgent need for early diagnosis and the search of new selective biomarkers. Selenium is an important constituent of selenoproteins and a powerful antioxidant able to protect against cancer. In this work, the absolute quantification of selenium in selenoproteins and the total content in selenometabolites has been performed for the first time in serum from lung cancer patients (LC) and healthy controls (HC). To this end, a method for the simultaneous speciation of selenoproteins using size exclusion chromatography (SEC) and affinity chromatography (AF) with detection by ICP-QQQ-MS, and quantification by isotopic dilution (IDA) (SEC-AF-HPLC-SUID-ICP-QQQ-MS) was developed to determine the selenium concentration in eGPx, SEPP1 and SeAlb, as well as total selenometabolites, to find alterations that may serve as biomarkers of this disease. In the same way, a method based on anion-exchange chromatography coupled to ICP-QQQ-MS was developed to quantify selenometabolites (SeCys2, SeMeSeCys, SeMet, selenite and selenate) in the same LC and HC serum samples. The results showed that the averaged concentrations of selenium in eGPx, SeAlb and selenite were significantly higher in LC patients (LC (eGPx: 21.24 +/- 0.77 ng g(-1); SeAlb: 49.56 +/- 3.16 ng g(-1) and Se(IV): 6.20 +/- 1.22 ng g(-1)) than in HC group (eGPx: 16.96 +/- 0.53 ng g(-1); SeAlb: 38.33 +/- 2.66 ng g(-1) and Se(IV): 3.56 +/- 0.55 ng g(-1)). In addition, the ratios between selenoproteins and selenometabolites have been calculated for the first to study their potential use as LC biomarkers. The rates eGPx/SEPP1, SEPP1/SeAlb, eGPx/Se(IV) and SEPP1/Se(IV) were significantly different between LC and HC groups. (C) 2020 Elsevier B.V. All rights reservedes_ES
dc.description.sponsorshipThis research was funded by the projects PGC2018-096608-B-C21 from the Spanish Ministry of Economy and Competitiveness, and P12-FQM-0442 from the Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain). Projects Neumosur (8/2012 and 9/2015) and SEPAR (124/2012 and 091/2016). Finally, authors are grateful to FEDER (European Community) for financial support, grants number UNHU13-1E-1611 and UNHU15-CE-3140.es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subject.otherLung canceres_ES
dc.subject.otherIsotopic dilutiones_ES
dc.titleAbsolute quantification of selenoproteins and selenometabolites in lung cancer human serum by column switching coupled to triple quadrupole inductively coupled plasma mass spectrometryes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/Spanish Ministry of Economy and Competitiveness PGC2018-096608-B-C21es_ES

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