Within the context of precision agriculture, goods insurance, public subsidies, fire damage
assessment, etc., accurate knowledge about the plant population in crops represents valuable
information. In this regard, the use of Unmanned Aerial Vehicles (UAVs) has proliferated as an
alternative to traditional plant counting methods, which are laborious, time demanding and prone
to human error. Hence, a methodology for the automated detection, geolocation and counting of
crop trees in intensive cultivation orchards from high resolution multispectral images, acquired by
UAV-based aerial imaging, is proposed. After image acquisition, the captures are processed by means
of photogrammetry to yield a 3D point cloud-based representation of the study plot. To exploit the
elevation information contained in it and eventually identify the plants, the cloud is deterministically
interpolated, and subsequently transformed into a greyscale image. This image is processed, by
using mathematical morphology techniques, in such a way that the absolute height of the trees
with respect to their local surroundings is exploited to segment the tree pixel-regions, by global
statistical thresholding binarization. This approach makes the segmentation process robust against
surfaces with elevation variations of any magnitude, or to possible distracting artefacts with heights
lower than expected. Finally, the segmented image is analysed by means of an ad-hoc moment
representation-based algorithm to estimate the location of the trees. The methodology was tested
in an intensive olive orchard of 17.5 ha, with a population of 3919 trees. Because of the plot’s plant
density and tree spacing pattern, typical of intensive plantations, many occurrences of intra-row tree
aggregations were observed, increasing the complexity of the scenario under study. Notwithstanding,
it was achieved a precision of 99.92%, a sensibility of 99.67% and an F-score of 99.75%, thus correctly
identifying and geolocating 3906 plants. The generated 3D point cloud reported root-mean square
errors (RMSE) in the X, Y and Z directions of 0.73 m, 0.39 m and 1.20 m, respectively. These results
support the viability and robustness of this methodology as a phenotyping solution for the automated
plant counting and geolocation in olive orchards.