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dc.contributor.authorLebrón, José Antonio
dc.contributor.authorOstos, Francisco José
dc.contributor.authorLópez López, Manuel 
dc.contributor.authorMoyá, María Luisa
dc.identifier.citationLebrón, J. A., Ostos, F. J., López López, M., ... Moyá, M. L. (2020). Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material. Pharmaceutics, 12(5), 482. DOI:
dc.description.abstractGene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)3]2+ complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixed with the phospholipid DOPE. A relation between the size and the molar fraction was found and a multidisciplinary study about the interaction between the metallo-liposomes and DNA was performed. The metallo-liposomes/DNA association was quantified and a relationship between Kapp and was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scattering and agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structure of the liposomes. L/D values corresponding to the polynucleotide’s condensation were estimated. In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells than for cancer cell lines, and a good internalization into cells. The latter as well as the transfection measurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability of the Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy.es_ES
dc.relation.isversionofPublisher’s versión
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subject.otherNon-toxic nanocarrierses_ES
dc.subject.otherSpecificity by cancer cellses_ES
dc.subject.otherGene therapyes_ES
dc.subject.otherTransfection processes_ES
dc.titleMetallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Materiales_ES

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