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dc.contributor.authorBracamonte, Enzo R.
dc.contributor.authorFernández Moreno, Pablo T.
dc.contributor.authorBastida Milián, Fernando 
dc.contributor.authorOsuna, María D.
dc.contributor.authorAlcántara de la Cruz, Ricardo
dc.contributor.authorCruz Hipólito, Hugo E.
dc.contributor.authorDe Prado, Rafael
dc.identifier.citationEnzo R. Bracamonte, Pablo T. Fernández Moreno, Fernando Bastida, María D. Osuna, Ricardo Alcántara de la Cruz, Hugo E. Cruz Hipólito, Rafael De Prado. Identifying Chloris Species from Cuban Citrus Orchards and Determining Their Glyphosate-Resistance Status. Frontiers in Plant Science, November 2017, Volume 8, Article 1977. ESSN: 1664-462X. DOI 10.3389/fpls.2017.01977es_ES
dc.description.abstractThe Chloris genus is a C4 photosynthetic species mainly distributed in tropical and subtropical regions. Populations of three Chloris species occurring in citrus orchards from central Cuba, under long history glyphosate-based weed management, were studied for glyphosate-resistant status by characterizing their herbicide resistance/tolerance mechanisms. Morphological and molecular analyses allowed these species to be identified as C. ciliata Sw., Chloris elata Desv., and Chloris barbata Sw. Based on the glyphosate rate that causes 50%mortality of the treated plants, glyphosate resistance (R) was confirmed only in C. elata, The R population was 6.1-fold more resistant compared to the susceptible (S) population. In addition, R plants of C. elata accumulated 4.6-fold less shikimate after glyphosate application than S plants. Meanwhile, populations of C. barbata and C. ciliata with or without glyphosate application histories showed similar LD50 values and shikimic acid accumulation rates, demonstrating that resistance to glyphosate have not evolved in these species. Plants of R and S populations of C. elata differed in 14C-glyphosate absorption and translocation. The R population exhibited 27.3-fold greater 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) activity than the S population due to a target site mutation corresponding to a Pro-106-Ser substitution found in the EPSPS gene. These reports show the innate tolerance to glyphosate of C. barbata and C. ciliata, and confirm the resistance of C. elata to this herbicide, showing that both non-target site and target-site mechanisms are involved in its resistance to glyphosate. This is the first case of herbicide resistance in Cuba.es_ES
dc.description.sponsorshipThis work was funded by the AGL2016-78944-R (MICINN, Spain), GR15112 (Research Group AGA001 - Junta de Extremadura, Spain) and 242088 (CONACYT, Mexico) projects.
dc.publisherUniversity of Bern, Switzerlandes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subject.otherShikimate-3-phosphate synthasees_ES
dc.subject.otherGlyphosate translocationes_ES
dc.subject.otherHerbicide resistance mechanismses_ES
dc.subject.otherPro-106 mutationes_ES
dc.subject.otherTall windmill grasses_ES
dc.titleIdentifying Chloris Species from Cuban Citrus Orchards and Determining Their Glyphosate-Resistance Statuses_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN, Spain [AGL2016-78944-R]

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