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dc.contributor.authorMontero Lobato, Zaida
dc.contributor.authorRamos Merchante, Adrián
dc.contributor.authorFuentes Cordero, Juan Luis
dc.contributor.authorSayago Gómez, Ana 
dc.contributor.authorFernández Recamales, María Ángeles 
dc.contributor.authorMartínez Espinosa, María Rosa
dc.contributor.authorVega Piqueres, José María
dc.contributor.authorVílchez Lobato, Carlos 
dc.contributor.authorGarbayo Nores, Inés
dc.identifier.citationZaida Montero-Lobato, Adrián Ramos-Merchante, Juan Luis Fuentes, Ana Sayago, Ángeles Fernández-Recamales, Rosa María Martínez-Espinosa, José María Vega, Carlos Vílchez,Inés Garbayo. Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology. Mar Drugs. 2018 Oct 9;16(10). ISSN: 1660-3397. DOI : 10.3390/md16100372.es_ES
dc.description.abstractHaloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50ºC), pH (4-10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81ºC, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 +- 0.64) and production of total carotenoids (3.34 +- 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 +- 0.98) and carotenoid production (3.74 +- 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea.es_ES
dc.publisherNational Center for Biotechnology Informationes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subject.otherHaloferax mediterraneies_ES
dc.subject.otherResponse surface methodologyes_ES
dc.subject.otherCentral composite designes_ES
dc.titleOptimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodologyes_ES

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