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dc.contributor.authorGonzález Domínguez, Raúl 
dc.contributor.authorGarcía Barrera, Tamara 
dc.contributor.authorVitorica, Javier
dc.contributor.authorGómez Ariza, José Luis
dc.identifier.citationGonzález Domínguez, R., García Barrera, T., Vitorica, J., Gómez Ariza, J.L.: "Application of metabolomics based on direct mass spectrometry analysis for the elucidation of altered metabolic pathways in serum from the APP/PS1 transgenic model of Alzheimer's disease". Journal of Pharmaceutical and Biomedical Analysis. Vol. 107, págs. 378-385. DOI: 10.1016/j.jpba.2015.01.025es_ES
dc.identifier.issn1873-264X (electrónico)
dc.description.abstractMetabolomic analysis of brain tissue from transgenic mouse models of Alzheimer's disease has demonstrated a great potential for the study of pathological mechanisms and the development of new therapies and biomarkers for diagnosis. However, in order to translate these investigations to the clinical practice it is necessary to corroborate these findings in peripheral samples. To this end, this work considers the application of a novel metabolomic platform based on the combination of a two-steps extraction procedure with complementary analysis by direct infusion electrospray mass spectrometry and flow infusion atmospheric pressure photoionization mass spectrometry for a holistic investigation of metabolic abnormalities in serum samples from APP/PS1 mice. A number of metabolites were found to be perturbed in this mouse model, including increased levels of di- and tri-acylglycerols, eicosanoids, inosine, choline and glycerophosphoethanolamine; reduced content of cholesteryl esters, free fatty acids, lysophosphocholines, amino acids, energy-related metabolites, phosphoethanolamine and urea, as well as abnormal distribution of phosphocholines depending on the fatty acid linked to the molecular moiety. This allowed the elucidation of possible pathways disturbed underlying to disease (abnormal homeostasis of phospholipids leading to membrane breakdown, energy-related failures, hyperammonemia and hyperlipidemia, among others), thus demonstrating the utility of peripheral samples to investigate pathology in the APP/PS1 model.es_ES
dc.description.sponsorshipThis work was supported by the projects CTM2012-38720-C03-01 from the Ministerio de Ciencia e Innovacion and P012-FQM-0442 and P009-FQM-4659. Raul Gonzalez Dominguez thanks the Ministerio de Educacion for a predoctoral scholarship (AP2010-4278)es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subject.otherAPP/PS1 micees_ES
dc.subject.otherAlzheimer's diseasees_ES
dc.titleApplication of metabolomics based on direct mass spectrometry analysis for the elucidation of altered metabolic pathways in serum from the APP/PS1 transgenic model of Alzheimer's diseasees_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/Ministerio de Ciencia e Innovacion [CTM2012-38720-C03-01]es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/Ministerio de Educacion [AP2010-4278, P012-FQM-0442, P009-FQM-4659]

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