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dc.contributor.authorCordero, Baldo F.
dc.contributor.authorCouso Liañez, Inmaculada
dc.contributor.authorLeón Bañares, Rosa María 
dc.contributor.authorRodríguez, Herminia
dc.contributor.authorVargas, María Ángeles
dc.date.accessioned2020-06-09T09:20:21Z
dc.date.available2020-06-09T09:20:21Z
dc.date.issued2012-09
dc.identifier.citationCordero, B. F., Couso Liañez, I., León Bañares, R., Rodríguez, H., & Vargas, M. A. (2012). Isolation and Characterization of a Lycopene ε-Cyclase Gene of Chlorella (Chromochloris) zofingiensis. Regulation of the Carotenogenic Pathway by Nitrogen and Light. Marine Drugs, 10(12), 2069–2088. DOI: https://doi.org/10.3390/md10092069es_ES
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10272/18230
dc.description.abstractThe isolation and characterization of the lycopene ε-cyclase gene from the green microalga Chlorella (Chromochloris) zofingiensis (Czlcy-e) was performed. This gene is involved in the formation of the carotenoids α-carotene and lutein. Czlcy-e gene encoded a polypeptide of 654 amino acids. A single copy of Czlcy-e was found in C. zofingiensis. Functional analysis by heterologous complementation in Escherichia coli showed the ability of this protein to convert lycopene to δ-carotene. In addition, the regulation of the carotenogenic pathway by light and nitrogen was also studied in C. zofingiensis. High irradiance stress did not increase mRNA levels of neither lycopene β-cyclase gene (lcy-b) nor lycopene ε-cyclase gene (lcy-e) as compared with low irradiance conditions, whereas the transcript levels of psy, pds, chyB and bkt genes were enhanced, nevertheless triggering the synthesis of the secondary carotenoids astaxanthin, canthaxanthin and zeaxanthin and decreasing the levels of the primary carotenoids α-carotene, lutein, violaxanthin and β-carotene. Nitrogen starvation per se enhanced mRNA levels of all genes considered, except lcy-e and pds, but did not trigger the synthesis of astaxanthin, canthaxanthin nor zeaxanthin. The combined effect of both high light and nitrogen starvation stresses enhanced significantly the accumulation of these carotenoids as well as the transcript levels of bkt gene, as compared with the effect of only high irradiance stress.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation.isversionofPublisher’s versión
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subject.otherCarotenoidses_ES
dc.subject.otherMicroalgaees_ES
dc.subject.otherLycopene cyclase geneses_ES
dc.subject.otherGene expressiones_ES
dc.subject.otherGene regulationes_ES
dc.titleIsolation and Characterization of a Lycopene ε-Cyclase Gene of Chlorella (Chromochloris) zofingiensis. Regulation of the Carotenogenic Pathway by Nitrogen and Lightes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.3390/md10092069
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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